Cellular Respiration Lab Report: Calorespiromerty Studies of Plant Respiration


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One of the major challenges for plant physiologists is to determine how plants acclimate metabolically to their environment. Certainly, many qualitative differences, including C3 and C4 photosynthesis, are commonly recognized, although there exist lots of differences that are difficult to measure due to their relatively fleeting appearance. Biologists who are acquainted with writing essays for botany know that standard measures of plant’s physiology are usually not useful for identifying the correlation between abiotic characteristics and physiological peculiarities of the plant, cellular respiration. However, the method of calorespirometry has shown its usefulness for measuring the respiratory properties of plant cells that remain indistinguishable for other approaches, thus proving its applicability for plant physiology.

Calorespiromerty as a Method

Nowadays, one can easily find calorespirometry among CSE paper presentation topics related to microbiology or animal physiology, but until recently, calorimetric methods have been employed exclusively for studying plans. Moreover, existing respiration models were limited by a lack of measuring methods for the substrate efficiency of carbon conversion in plants. There are large amounts of data related to respiration rates, but only scarce data related to directly measured efficiencies. Some authors claim that single measures of CO2 production and O2 uptake rates are correlated with plant growth rates. But this knowledge remains insufficient as long as we know little about how the relationship between growth and metabolism changes with experimental conditions. It is not possible to find such calculations in books with an algebra homework help.

Calorespiromerty is a convenient method that permits to measure both rate and efficiency of plant metabolism because it allows simultaneous measurement of these parameters as functions of temperature. Although, it requires building a model based on both thermodynamics and plant biochemistry. Otherwise, it would be but nothing special – just one more method for measuring respiration rates, even though an especially useful one. Nevertheless, from writing college application essays to establish international collaborations, any genuine scientist is much stronger interested in an accomplished theory than in a simple enumeration of methods. That is why the usage of calorespirometry in modern plant physiology tends to develop towards the fundamental understanding of how energy from respiration is employed in moving nutrients and photosynthate into new tissues.

Specifics and technological difficulties of methods for calorespiromerty studies

There exist some peculiarities of calorespiromerty which should be concerted with a cellular respiration lab report format. These specifics are as follows:

Selection of material

Practically, any live plant material can be studied by calorespirometry, as the sample can fit into the measuring chamber. Two types of calorimeters directly measure instantaneous heat rates: power compensation and heat conduction calorimeters. Both of them can employ measuring chambers in a range from 1 cm3 to room size, and therefore, can be used for the determination of metabolic rates of plants and organisms of almost any sort and kind. Thus, there have been conducted studies ranging from single plant cells to tissue sections or entire seedlings and even whole heads of cauliflower. However, studying huge samples takes an enormous amount of time – much more time than one needs for finding a College Test Preparation homework help – so it is more convenient to do measurements in small chambers up to an ampoule size.

Conditions of measurement

Normally, studies of plant tissue culture require sterile conditions. Other tissues don't need to be sterile. They only need to be clean from pathogenic fungi and bacteria. Usually, microbial decontamination presents no difficulties when it goes to leaf and stems tissue. However, there could be problems with roots because the process of taking the roots is generally complicated by contamination from the growing medium. That is why any good cellular respiration lab report example will recommend you using the sand culture of roots, which produces samples with relatively low levels of contamination. Finally, the investigator usually has from two to four days for transportation of plant tissue cuttings, since such cuttings are stable enough not to undergo significant changes in respiratory characteristics in a couple of days.

Stabilization of heart rates

The method of calorespiromerty envisages sealing ampoules with samples to prevent water loss. Unfortunately, the build-up of toxic products and temperature sensitivities can cause instability in heat rates. Also, some plant species' wound responses can contribute greatly to the phenomenon of rapidly changing heart rates. Planning a biological experiment has nothing in common with composing restaurant business plans, and there are many things one could not foresee. However, it is possible to check the risk of the wounding response by simply comparing a tissue cut once with the sample of the same tissue cut multiple times.

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