Cloning and Gene Therapy essay

Digest two to five microgram of vector DNA using restriction enzymes needed for the DNA insertion. Secondly, prepare insert DNA for cloning –insert DNA can alternatively be prepared with the use of restriction enzymes, This is through the use of T4 DNA ligase enzymes, vector and insert DNA can be ligated. The last step involves the competent cell preparation –this can be done using heat shock method or calcium chloride treatment or through the use of electrophoresis (Simon et al. Gene therapy is the healing or treatment with the use of genes. Cloning is involved in gene therapy whereby the gene from the affected cells can be removed in-vitro that is outside the body and to these cells a desired or correct gene can be inserted.