Effects of Temperature and pH on Catalase Enzyme Activity in Liver and Banana Using H2O2 Student Name: Department: School: Institution: EFFECTS OF TEMPERATURE AND pH ON CATALASE ENZYME ACTIVITY IN LIVER AND BANANA USING H2O2 ABSTRACT The purpose of this experiment is to examine effects of temperature and pH on activity of catalase enzyme in Banana and Liver using hydrogen peroxide. Catalase enzymes are found in both animal and plant tissues. Both low and high temperatures slow down catalase activity. Very high temperatures denature catalases leading to no activity. Low temperatures inactivate enzymes leading to slow activity. Increased surface area increases catalase activity and therefore increased oxygen production. Both acidic and basic pH slow down catalase enzyme activity. A neutral pH of 7 offers an optimum catalase activity. INTRODUCTION Catalase enzyme catalyzes hydrogen peroxide decomposition to form water and oxygen CITATION Che04 \l 1033 (Chelikani Loewen & Fita 2004). for all enzymes. References BIBLIOGRAPHY Alberts B. Lewis J. Johnson A. Raff M. Roberts K. & Walter P. (2002). "Peroxisomes". Molecullar Biology of the Cell (4th ed.). New York: Garland Science. Boon E. M. Downs A. & Marcey D. (2007). Proposed Mechanism of Catalase. Catalase Structural Tutorial. Chelikani P. Loewen P. C. & Fita I. (2004). Diversity of structures and properties among catalases. Celular and Molecular Life Sciences 192-208. Ilyukha V. A. (2001). Superoxide Dismutase and Catalase in the organs of mammals of Different Ecogenesis. Journal of Evolutionary and Biochemistry and Physiology 241-245. Loew O. (1900). A New Enzyme of General Occurrence in Organism. Science 701-702. Putnam C. D. Arvai A. S. Bourne Y. & Tainer J. A. (2000). Active and inhibited human catalase structures: ligand and NADPH binding and catalytic mechanism. Journal of Molecular Biology 295-309. Summer J. B. & Dounce A. L. (1937). Crystalline Catalase. Science 366-367. [...]
This report will be based the data and graph i attached below, it will measure the amount of oxygen produced at various temperatures and pH. i want this 100% plagarigrized free. It should be about 6 pages. All the graphs and tables should be presented accordingly with writers own knowledge. I also have attached Lab requirments file the report should follow all the criteria as written on lab instructons.