Microscopy Gram staining

Their cell structure constitutes of peptidoglycan, lipids and proteins and the three vary in amounts in the cell of each bacteria. In gram staining technique, the method stains peptidoglycan contained in the cell structure. The results of the procedures display different colors upon staining. A higher percentage of peptidoglycan (gram positive) in the cell structure stains purple whereas a lower percentage of peptidoglycan (gram negative) stains red or pink. This paper sets to present the laboratory results obtained by a group of three students upon following the set procedures and observations taken. PH changes on addition an acid or an alkali thus a buffer will resist these changes upon addition insignificant amounts of these substances. The work presented below is the laboratory preparation and results obatained.

Pipetting principles. In the air displacement pipette, the air between the liquid and the pipette and thus when the air is drawn the liquid is drawn too and on releasing the air the liquid is dispensed. (lennette p. The amount needed is only 500mls therefore, 1L= 1000mls an for 500mls the amount rof tris required is given by; 60. 275g. 275g of tris. Weight of NaCL; The saline is 0. 9% to make 100mls of saline 0. 1M=1000mm To make a solution of 20mm, you divide 1000 by 50 therefore, the solution of 20mm the amount added is equivalent to 121. 1g/50= 2. 422g. The amount needed is only 500mls therefore, 1L= 1000mls an for 500mls the amount rof tris required is given by; 2. 211g. 9g of NaCL is added to 100mls therefore raising the amount to 500mls the amount added is five times the 0.

9g, that is 0. 5g. This gives 4. 5g of NaCL to be added to make the solution. Preparation of 70%v/v ethanol. Amount of water and ethanol required. For 100ml we have 70mls of ethanol and 30mls of water. For 50ml, the value is smaller by 2 therefore we divide by 2. 70/2= 35mls of ethanol and 30/2 =15 mls of water. The underlying principle of this technique is that there a variance in wavelength for each compound absorbing light. procedure of making the dilutions. Required. 2mg/ml BSA and distilled water. Results and calculations. The process is determined by the penetration of the mobile electropholical particles through the gel. Personal development profile Part 1: skills evaluation. Asses your skills in the below mentioned ranges. 1= not proficient. 2= less proficient.

First and last name Date Academic title and rank Professional goals for this year. 1- Getting aquinted with mor and more knowledge in biomedine. 2- Developing new ideas and increase creativity in my field. 3- Bettering oral and manuscript presenation skills. Motivating factors. Achieved previous goals. i. Analytical skills have been able to grow better to a proficient level ii. Gettting along with others was also achieved as now I can perfectly get along with all the collegues. Time management Previous year Next year % time on patient care 50% 25% % time on research and creative work 33% 45% % time on training 17% 30% Total time. Kaplan LA, Pesce A. J, Kazmierczak SC Clinical Chemistry: Theory, Analysis and Correlation. Mosby, 3, (6) (2003). Reed R, Holmes D, Weyers J and Jones A (Practical Skills in Biomolecular Sciences.