Efficiency of different methods of bacterial transformation

The main aim of this experiment was to calculate the efficiency of bacterial transformation in E. Coli. The main use of plasmid DNA in the experiment is because it contains some traits that are required for bacterial survival in relationship to the variables and controls required in the experiment. SOC maximizes the transformation efficiency of competent cells. It was identified that there were high rates of transformational efficiency. Materials and Methods The electroporator cuvettes were chilled and the bacteria cells gently thawed by placing them on ice. microliters of the bacterial cell were transferred to the tube labeled E. Coli to the electroporator cuvette. ng of plasmid DNA were then added to the electroporator cuvette and mixed with the cells by tapping the bottom of the cuvette.

The metal sides of the cuvettes were gently wiped, and the cuvette placed in the electroporator, the lid closed, and the red button pressed till the electroporator beeped. Therefore, bacteria that showed light under the UV light would have the plasmids and transformation would have been successful to the desired gene, thus surviving in Agar Plate B, expressing the GFP gene. Therefore: SOC =300ul CC=40ul SOC used was 250ul  Number of colonies = 70 1ul plasmid+300ul SOC+40ul CC = 341 341/250 = 1. TE= (70*1. Each colony represents one bacteria that has been transformed. Using this the efficiency can be determined (Gilbertson, 2017). Claverys, J. P. Bacterial transformation: distribution, shared mechanisms and divergent control.  Nature Reviews Microbiology, 12(3), 181. Okshevsky, M.