UNKNOWN BACTERIA IDENTIFICATION REPORT Name: Partner’s name here Bio205 – Microbiology Section 0# HB Professor Olken Abstract This experiment involved the identification of unknown microorganism using different identification tests. The experiment employed a dichotomous key which incorporated several tests that helped narrow down to the identity of specific microorganisms which were unknown. The tests used included; grams staining culture method PHR lactose catalase test motility deep citrate phenyl alanine deaminize test mannitol urea slant and catalase test. The results showed that the two unknown microorganisms 1 and 4 were S. aureas and Proteus mirabilis respectively and these microorganism cause infections in the human body. These identification techniques are important in hospitals and research centers in the diagnosis of diseases before medications area provided. Introduction Diagnostic tests are tests done on a sample to determine the microorganism present based on its characteristics which might be physical or chemical. For example right medication. In future more time should be given during the semester to allow collection of better results. References Lab Manual adapted from Guilmette N Kadambi B Quach H. Methods in Microbiology 3rd edition. McGraw-Hill. 2011. Feng Q. L. et al. "A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus." Journal of biomedical materials research 52.4 (2000): 662-668. Centers for Disease Control and Prevention (CDC. "Staphylococcus aureus resistant to vancomycin--United States 2002." MMWR. Morbidity and mortality weekly report51.26 (2002): 565. Coudron Philip E. Ellen S. Moland and Kenneth S. Thomson. "Occurrence and detection of AmpC beta-lactamases among Escherichia coli Klebsiella pneumoniae and Proteus mirabilis isolates at a veterans medical center." Journal of Clinical Microbiology 38.5 (2000): 1791-1796. Nugent Robert P. Marijane A. Krohn and Sharon L. Hillier. "Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation." Journal of clinical microbiology 29.2 (1991): 297-301. [...]
Identification of Two Unknown Bacteria LAB REPORT GRADING: Your grade depends on your following these instructions in writing the report, your laboratory technique (good aseptic practices), following of each procedure correctly, complete record of results, proper interpretation and explanation of data. General grading (refer to your instructors rubric for more details) 10 points Lab Notebook: All information is present, organized, and carefully documented for each week of the lab project. Brief interpretations of results are present. 20 points Identification: The two bacterial species have been correctly isolated and identified from the list of possible unknowns. 10 points Lab Report Formatting: Report follows correct lab report formatting. (See attached.) All the information is in the correct section. Reference(s) are properly cited. 5 points Grammar and Spelling: Report has good grammar and spelling. (Get a Proofreader!) Use italics for Genus and species. (Ex. Clostridium difficile ) 5 points Title and Abstract Section: Title is appropriate and specific (“Unknown Lab” is a poor title) Abstract is a brief but thorough overview that states the number and identity of each unknown. 5 points Introduction Section: Introduces the topic and states the importance of the topic for the reader. Introduction should not contain any methods, results, or discussion. 5 points Methods Section: Methods section briefly describes all methods used in a logical manner. Materials and details of your methods are not required. 10 points Results section: Clearly presents all results organized in typed table(s). Tables are referenced in a short descriptive (non-analytic) text. 25 points Discussion section: Your conclusions are defended in a logical argument. How did you narrow down the list? What tests led you to reject other bacteria? Writing “the results matched Bergey’s” isn’t good enough! 5 points Review (in Discussion) section: Did any of the tests give inconclusive results? Given more time, would you have repeated any tests? Were any errors made in your technique? If yes, how could these have been avoided? NOTE: Plagiarism will result in a failing grade. If you are uncertain if you have plagiarized, check with your professor. It is not worth the risk!