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Chemistry Assignment

Five standards that contained know concentrations of protein were prepared. The spectrophotometer was set at 280nm and used to determine the absorbance of each of the samples. The values are below. The purpose of performing these tests was to prepare a standard curve (in this case the “curve” is expected to be a line) that can be used to determine the amount of protein in a sample. Use Excel to plot this data to develop the standard curve. Be sure to provide an appropriate title (not x versus y), label all axes with values and units, have the graph on its own ‘sheet,’ have Excel generate the best fit line for the data, include the equation for the line, and give the R-squared value for the line. Also include an additional sheet with the values below. This sheet will be used to verify that your values on the graph are congruent with the values from the assignment. You will then need to submit this file on Blackboard as an Excel file. This assignment is due before the beginning of the next lecture meeting. Late assignments will result in half credit. Concentration (mg protein/L) Absorbance at 280nm 0.000 0.000 0.425 0.236 0.897 0.457 1.279 0.699 1.692 0.974 2.195 1.192
Five standards that contained know concentrations of protein were prepared. The spectrophotometer was set at 280nm and used to determine the absorbance of each of the samples. The values are below. The purpose of performing these tests was to prepare a standard curve (in this case the “curve” is expected to be a line) that can be used to determine the amount of protein in a sample.
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Assignment ID
23983
Discipline
Type
CREATED ON
April 26, 2016
COMPLETED ON
April 28, 2016
Price
$40
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