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Biology - BIOTECHNOLOGY , Coursework

Task Four: Part 1 Prior to CRISPR-Cas9 editing of genes, two other techniques were developed. The first utilizes zinc-finger nucleases and the second utilizes TAL effector nucleases and is known as TALEN. These techniques are still being developed and have their own advantages and disadvantages. In your own words, BRIEFLY describe each technique. Hint: use Pubmed to search for reviews of gene-editing nucleases. Task Four: Part 2 The anti-retroviral drug EFAVIRENZ is used in the treatment of HIV infections but within the initial weeks of treatment about 50% of patients suffer CNS side effects including dizziness, insomnia and depression. Plasma clearance of the drug was noted to be markedly less in African Americans than in European Americans. Use the OMIM (http://omim.org) database as a starting point to answer the following questions: 1. What gene is primarily associated with variations in metabolism of this drug? 2. What is the SNP in this gene that has been identified as being responsible for the difference in drug metabolism between African Americans and European Americans? 3. What percentage of homozygosity of this SNP was found in African Americans versus European Americans? 4. Follow the link to the entry for this SNP on the e!Ensemble Asia database (http://asia.ensemble.org) and then look for the Population Genetics of this SNP to find out what percentage of Asian people have this SNP.
Task Four: Part 1 Prior to CRISPR-Cas9 editing of genes, two other techniques were developed. The first utilizes zinc-finger nucleases and the second utilizes TAL effector nucleases and is known as TALEN. These techniques are still being developed and have their own advantages and disadvantages. In your own words, BRIEFLY describe each technique.
Assignment ID
20915
Discipline
CREATED ON
10 April 2016
COMPLETED ON
12 April 2016
Price
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10 April 2016
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10 April 2016
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10 April 2016
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12 April 2016
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