Nowadays, the use of DSN or duplex-specific nuclease normalization methods to whole-genome shotgun of genomes with a high percentage of repetitive DNA should be studied by students. Basically, this interesting method utilizes the thermostable DSN that hydrolyzes genomic DNA. There are endless model experiments of people’s genomic DNA, and their basic purpose is to demonstrate that the normalization of double-stranded DNA that is formed during the C0t analysis can be efficient against all kinds of repetitive sequences with their high-sequence identity. Another great thing is that it can retain its coding regions and highly divergent repeats at a base-line level.
This means that this type of DSN normalization applied to the above-mentioned analysis can be widely used to get rid of unwanted evolutionarily young repetitive components of genomic DNA before its sequencing. Besides, this method proves to be invaluable in different studies of such large eukaryotic genomes as the ones that belong to higher plants. WGS or whole genome shotgun is quite an effective strategy when studying reference sequences in different genomes. The main reason is that it helps people generate the necessary sequences data, thus resulting in overlapping these sequences eventually.
Those aligning DNA sequences that can achieve their overlapping sequence tend to gather into the so-called contigs that are easy to read by special computer programs. However, this method can’t be applied if any redundant repetitive sequences exist in big genomes. There are other effective methods, including MSLL and MF, which can be used to eradicate redundancy in the genomes of higher plants when they depend on the hyper-methylation tendency of these repetitive sequences.
In addition, the usage of genomic libraries or enzymes can potentially modify genomes, but it can be applied only to their limited part. Many talented scientists offer another efficient method to achieve the same goal, and they call it high-temperature DNA analysis. It follows specific DNA renaturation kinetics and use denatured, sh4eared, and re-anneled genomes. It’s worth noting that this field is quite new, so it’s still open to all kinds of innovative findings, methods, theories, etc. The normalization of genomic DNA via duplex-specific nuclease is not different.
Nowadays, the use of DSN or duplex-specific nuclease normalization methods to whole-genome shotgun of genomes with a high percentage of repetitive DNA should be studied by students. Basically, this interesting method utilizes the thermostable DSN that hydrolyzes genomic DNA.