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Two substances from cultivation of the endophytic fungus Penicillium sp of leaves of kunyit putih (Curcuma zedoaria (Berg) Roscoe ) have already been isolated. The endophytic fungus was cultivated on 5 L of Potatos Dextose Broth (PDB) moderate at room heat range (no shaking) for 3 weeks. The cultures were after that extracted with ethyl acetate to cover 3.0 g of residue after removal of the solvent under reduced pressure. The extract was separated and purified by silica gel column chromatography (CC) and afforded two pure substances as yellow oil (substance 1) and yellowish crystal ( substance 2). The structure of the substances were established by comprehensive UV, IR and NMR spectroscopic analyses and substance 1 along with evaluation with the reported data. Base on spectra analyses the compound 1 was determined as Di-(2-ethylhexyl)phthalate and compound 2 as 5 (4вЂ™-ethoxy-2вЂ™-hydroxy-5вЂ™-methyl-2вЂ™,3вЂ™-dihydrofuran-3вЂ™-il (hydroxy) methyl-4-isopropyl-3-methyl-2-pyran-2-on). Compund 1 isn't new compound, nonetheless it is fresh for endophitic fungus from Curcuma zeodoria and substance 2 is new substance. Endophytic microorganisms that redise in the tissues of living plants and could produce secondary metabolites of biologically active . Novel antibiotics, antimycotics, immunosuppressants, anticancer compound are just a few types of what has been discovered after the isolation, culture and purification and characterization of some choice endophytes recently. isolation of their bioactive secondary metabolites of endophytic fungus from plant could possibly be selected mainly something on ethonobotanical history. Curcuma zedoaria, a medicinal tuber plant owned by the grouped family members Zingiberaceae has been found in the original system of medicine.