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Mushrooms is exclusive creature in the living globe cannot be categorized neither plant nor pet. It has become among human food sources. There's been recent finding of curiosity in mushroom not merely as a health veggie which abundant with protein but it addittionally important as resources of biologically active substance of medical value. Predicated on Ooi & Liu (2000) schizophyllan from S. lentinan and commune, an interferon-stimulating polysaccharide made by L. edodes are items which have been isolated from mushroom to create anti-cancer drugs. Today, mushroom cultivation is becoming one of agronomic producing activity which may be applied to the tiny industry farmer. Therefore to be able to cultivated the mushroom there are many steps referred to as preparing medium lifestyle, spawn preparation, moderate substrate planning, spawning, spawn working, fructification and harvesting (make reference to the Amount 1 in Appendix 1). The first guidelines in mushroom cultivation are planning of culture mass media. Mushroom mycelium needs nourishment to develop. Agar (seaweed) contains minimal nutrition, but works as a gelling agent when blended with water, to ensure that the mycelium includes a flat, solid surface area to grow. A mixture of agar, drinking water, and nutritional substance provides satisfactory way for growing out healthful mycelium (Ogden & Prowse, 2004). The mycelium grows on the top of medium will later be utilized to inoculate larger levels of substrates like grain. Test tubes or Petri meals used as lifestyle containers. Young and vigorous mycelium obtained from a fruiting body of a mushroom. The mycelium ought to be white and grow right out of the tissue. If yellowish, blue, green, or grey mycelia form on other areas on the surface, then they are fungal contaminants. A cre...