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Isolation of SDS-degrading Pseudomonas aeruginosa sp

Assignment id 1004051
Discipline Arts Entertainment
Assignment type Essay
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characterization and characterization and characterization and

Isolation of SDS-degrading Pseudomonas aeruginosa sp. Department of Biochemistry, Faculty of Biomolecular and Biotechnology Sciences, University Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Abstract: Surfactants are synthetic organic chemicals that are formulated to have cleansing or solubilisation properties. With the development of the commercial increase and economy in population density, surfactants have become probably the most disseminated xenobiotics to get into the aquatic environment broadly, creating a significant environmental problem. Their toxicities to organisms have already been demonstrated previously. The main objective of the study was to isolate and characterize local bacteria with the potential to degrade Sodium Dodecyl Sulphate (SDS), a utilized anionic surfactant widely. Screening was completed by the traditional enrichment culture technique and the isolate was tentatively determined as Pseudomonas aeruginosa sp. using BiologTM GN plates and partial 16S rDNA phylogeny. The perfect growth circumstances in minimal moderate and for degradation of SDS by Pseudomonas aeruginosa sp. were at 30В°C and at 6 pH.5 using phosphate buffer system. Sodium nitrate; at 8.0gL-1 was discovered to be the very best nitrogen resource. The isolated stress exhibited optimum development at SDS focus of 1gL-1 but can tolerate up to 14gL-1 SDS, indicating that isolate could survive in a higher concentration of SDS fairly. 100% of just one 1.0gL-1 SDS was completely degraded after 5 and 2 times of incubation before and after optimization respectively. Keywords: SDS, biodegradation, MBAS assay, Pseudomonas aeruginosa sp. 1.0 Intro Anionic surfactants are sets of xenobiotic compounds which contain either sulfonated or e...

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