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Isolated Simple Muscle Contraction Experiment

Background information on smooth muscles

Smooth muscle is one of three muscle fiber content types found in pets. Unlike skeletal and cardiac muscle cells, smooth muscle skin cells are not striated, and have single nuclei. Soft muscles are typically under control of the autonomic nervous system, and don't contract voluntarily. Soft muscle contracts slowly, and does not exhibit the characteristic twitch observed in skeletal muscle. In addition, soft muscle is not prone to muscle fatigue, which makes it an ideal element of sphincter muscles. Even muscle is situated in the gastrointestinal tract of several animals, and is also responsible for peristaltic actions.

Smooth muscle contractions are influenced by calcium mineral and potassium ions. Calcium ion influx into the easy muscle cell initiates a contraction. Potassium ion attention in the extra cellular medium impacts the resting membrane probable of the cell, getting it closer to or farther away from its threshold voltage. Neurotransmitters have an impact on different kinds of smooth muscle differently, depending on connection of the soft muscle with excitable skin cells. Generally, acetylcholine increases the muscle cell's permeability to calcium, while epinephrine decreases the cell's permeability to calcium mineral.

Introduction and goal of the experiment

The following record was to check a clean muscle that was collected from the intestine of a rabbit. The main of this experiment was to see how the encompassing environment of the muscle could influence how muscle contracted. The test contains different conditions and the muscle was monitored and results were noted of the amplitude and the regularity of the wavelengths. The degrees of muscle contractions and relaxations were assessed by using a transducer, a D. C amplifier, and a laboratory computer.

Overview of experiment

The clean muscle was a tiny area of the intestine which was prepared effectively by trimming from the attached mesentery and extra fat. This test only required one piece of this easy muscle which was then put into a beaker that was aerated and fed Ringer-Locke solution this can be an aqueous solution containing the chlorides of sodium and potassium and calcium mineral that is isotonic to dog tissues. The test only required one little bit of small intestine, which had the distance of 2-3 cm long. The intestine happened set up with a structure holder, and was attached to the transducer via a piece of string. The transducer recognized contractions and rest of the muscle, and via the D. C amplifier showed on the computer the regularity and power of the contractions and relaxations. The perfect solution is that the muscle was placed in was maintained at the temps of 37 Celsius apart from when the environment condition changed was the heat range. This type of setup is known as 'in vivo' planning. This preparation of the simple intestine allows a precise control of environmentally friendly conditions.

Brief explanation of test transported out

The test that was carried out acquired six parts to it. The first area of the experiment was to gain preliminary control of the muscle this is done by having maximum conditions for the clean muscle so it could achieve a steady rate of contraction and rest. This was attained by adding Ringer-Locke solution and keeping it aerated. With this set up it allowed the muscle environment to be very carefully met to the perfect body environment where the muscle would have a good contraction and relaxation rate. The muscle was left in this prep before readings using the pc were frequent (about 4 minutes) the original control was labelled using the comment bar.

The next environment was non-aerated this designed to turn the air supply off that was coming to the bath where the muscle was held. Then your Ringer-Locke solution was removed from the bath and fresh Ringer-Locke solution was then put into the bathroom. The explanation for eliminating the old solution was to prevent any inaccurate readings as the solution could still have comprised oxygen which would have damaged the results. This part of the test was saved after each 5, 10, a quarter-hour and the results where put into a stand. The main purpose of by using environment was to see the particular muscle contractions and relaxations are when there is a lack of oxygen. This part of the test was again labelled on the comment club.

The next area of the experiment was to remove the Ringer-Locke solution and replace it with 50ml of glucose free solution and again results were noted after each 5, 10, quarter-hour and recorded into a desk.

The fourth different environment was change in heat. The Ringer-Locke solution of 37 Celsius was substituted with a Ringer-Locke solution that was cooled to 4 Celsius. The goal of this environment was to check the muscle activity in a frigid environment and also to analyse the effects.

The fifth environment engaged the utilization of calcium mineral free solution this changed the Ringer-Locke solution. This was analysed for 5 minutes to see how the muscle activity was infected.

The last environment was to do with changes in the pH. The pH was modified from pH7 which is natural to a new pH. The answer with different pH was ready in advance and the purpose of this environment was to see what impact a pH change would have on the muscle activity.

After every part of the experiment preliminary control was founded before shifting to another part the reason for this being to keep carefully the muscle jogging properly before each area of the experiment also to cause less damage to the soft muscle. Also every part of the experiment was labelled on the comment club this was done showing each different part clearly so it was not baffled. (Clear methods are shown in the printouts)

The clean muscle in the control environment was able to obtain a stable frequency through its 4 minute period with 15 waves per minute. The waves which were seen were the smooth muscle contracting and relaxing. The amplitude degrees of the waves were both quite high showing strong contractions rate the reason behind this is that the surroundings set in place was to ideal conditions where the muscle could perform its best. It possessed a utmost amplitude of 2. 10 and min amplitude of 0. 78 which ultimately shows that having ideal conditions the muscle is able to behave normally with no problems.

The aftereffect of oxygen lack of the soft muscle cause the tiny intestine to improve the frequency, at five minutes no aerated the frequency had first lowered to 13 and after 15 minutes the regularity of contraction and relaxations acquired increased to 17 the reason why or this being without air the clean muscles began to have spasms as it was struggling to contract properly with no oxygen resource needed. Also the amplitude levels lowered quite quickly from 1. 94 (maximum) and 0. 47(min) at five minutes to 1 1. 58(maximum) and 0. 38 (min) at 15 minutes. The explanation for this is that without oxygen the muscle was unable to make the energy necessary for strong contractions as the peak is reduced as can be seen on the traces. This is recognized by the move on the string that was mounted on the transducer; the move had not been as strong which means this was documented on the traces. So without oxygen the muscle cells are still in a position to make ATP but a little amount. No more than 2 ATP are produced per molecule of glucose in glycolysis. When there is no oxygen present, the Pyruvate stated in glycolysis goes through fermentation to regenerate the NAD+ used in glycolysis. This is known as anaerobic respiration, anaerobic respiration creates only two ATPs, and lactic acid solution is produced. Most lactic acid solution diffuses from the cell and into the bloodstream and is also subsequently absorbed by the liver. A number of the lactic acid remains in the muscle fibers, where it plays a part in muscle exhaustion. Because both liver and muscle fibers must convert the lactic acidity back to pyruvic acidity when air becomes available, anaerobic respiration is said to produce air debt

The next part of the experiment was to test the way the muscle activity differed when positioned in glucose free solution. Out of this part of the test the consistency of muscle activity remained constant throughout the 15 minutes. The traces show consistent movement as well as the amplitudes levels differed as at five minutes (1. 34) the utmost amplitude was low then at 10 minutes (1. 51) it wet higher with a quarter-hour (1. 37) it reduced again to a similar figure that was at five minutes. By looking at this result the results are not as correct as they should have been, meaning they could have been some kind of inaccuracy when following the method as without blood sugar, ATP can't be made and the amplitude of the waves should have been lower.

ATP can be made from blood sugar which is stored in the carbohydrate glycogen. From the fat burning capacity of glycogenolysis, glycogen is broken down to release sugar. ATP is then produced from glucose by cellular respiration. Also ATP can be created from glucose and fatty acids obtained from the bloodstream. When energy requirements are high, sugar from glycogen stored in the liver and fatty acids from fat stored in adipose skin cells and the liver organ are released in to the bloodstream. Glucose and essential fatty acids are then assimilated from the bloodstream by muscle skin cells. ATP is then generated from these energy-rich molecules by cellular respiration. Without sugar the frequecny should hve increased but the amplitudes levels should have decreased as there is not a sufficient power source which could supply the muscle so that it could written agreement and relax.

The next area of the experiment consisted of placing the even muscle into a cooled solution of 4 Celsius from an alteration of 37 Celsius. There is not much change to the rate of recurrence but it did drop a bit because of the muscle not being used to these environmental conditions. The amplitude differed from the control readings as they had reduced but were still quite high as that they had the nutrition in the solutions which helped those to contract. If this test was left to transport on then there would be further change as the answer would gradually warm up to room temperature and this would mean that the simple muscle activity would increase.

Calcium plays a major part in all muscle contraction as well as smooth muscle contraction which differs as it does not contain troponin. In even muscles calcium ions enter in from beyond your cell. Then they bind for an enzyme complex on myosin; this then breaks up ATP into ADP and then exchanges the Pi directly into myosin. Using this method it allows the myosin to trigger and from cross ridges with actin. When the calcium mineral is pumped from the cell, the Pi gets taken off myosin by an enzyme this enables the myosin to be inactive and the easy muscle can relax. This process is also known as myosin controlled contraction.

In the test where calcium free solution was added it afflicted the simple muscle greatly as the frequency of contracting and soothing fallen to 12. Also the amplitude levels came down as the contractions and relaxations levels were not strong the utmost being 0. 95 and the least being 0. 47 if this is left for an extended time period the regularity levels may have dropped more. Without calcium joining the cell the clean muscle struggles to do the procedure which is identified above. While making the Ringer-Locke solution it isn't only the calcium mineral ions which are important to the even muscles. Some others are potassium chloride and sodium chloride, the reason why these ions are needed since it helps to portray a host like the body with ideal conditions. Only if distilled drinking water was used it could mean the cells in the muscles would up take the normal water and blow up. So these ions are being used so they are able to keep a concentration gradient and allow everything to work correctly as it would in the simple muscles natural environment.

The final environment was the change in pH levels. The results showed that the muscle got stopped working and there was no reading on the traces. This intended that there is no muscle contraction or relaxing. The reason for this is the muscle possessed broken down the reason for this muscle fatigue was that the reduced pH had afflicted the clean muscle as it was struggling to perform in this kind of environment. Also as the low pH solution was there for a certain time frame the muscle was struggling to remove it and for that reason induced the muscle to malfunction. The reduced pH may have afflicted the sarcoplasmic reticulum which may lead to the interfere of the intercellular calcium concentrations, this may lead to long term physical muscle damage as muscle materials are afflicted.

While planning the isolated clean muscle many safeguards are taken so the muscle can avoid abnormal pH changes. Among these precautions which can be taken is to be sure that before placing the tiny intestine in to the bath. The perfect solution is should be examined with pH signal test strips that will give a fairly exact reading of the solution which the smooth muscle will be positioned. This is very important as if the pH is incorrect it will mean that the clean muscle will not perform to its full potential meaning the results achieved will be inaccurate. To gain exact results all solutions which are being used will need to be inspected to see if they are the right pH by using the ph indicating pieces. Following this test the smooth muscle was unable to reach the control again as the muscle experienced broken down.

If this test was done again at room temperatures the results would change as the performance of the muscle would decrease. This is because the optimum temp inside the body is just about 37 Celsius which heat range allows the muscle to just work at an most effective rate. The low temperature will mean that muscle contraction will be slower as you will see an effect on enzyme reactions as a lot more heat there is the more kinetic energy there is certainly this means that the muscle activity will be good.

If acetylcholine was put into the solution bathtub of the even muscle the membrane potential would reduce and the occurrence of waves would increase. The muscle can be more active, with a rise in tonic pressure and the amount of rhythmic contractions. The effect is mediated by enzymes which escalates the intracellular Calcium focus. Another substance that could have been added was adrenaline. Adrenaline allows bloodstream to flow easier to muscle tissue. This means that more air is carried to the muscles by the extra blood, that allows your muscles to operate at raised levels. Adrenaline also helps the change of your body's gasoline source (glycogen) into its gasoline (sugar). This carbohydrate offers energy to muscles, and an abrupt burst of sugar also allows muscles to bolster further.

Skeletal and easy muscle muscles distinctions in composition and function

There are many differences between your two types of muscles the distinctions are mentioned below:

Smooth muscles

  • A smooth muscle fiber has no T tubules, and the sarcoplasmic reticulum varieties a loose network throughout the sarcoplasm.
  • Are long cyrindrical cells which contain many nuclei
  • Smooth muscle mass has no myofibrils or sarcomeres
  • They are striated this shows their specific alignments of their myofilaments.
  • This tissue also offers no striations and is called nonstriated muscle.
  • Thick filaments consist of myosin as slim filaments consist typically of actin.
  • Thick filaments are spread throughout the sarcoplasm of your easy muscle cell
  • Each 3rd party cell is stimulated by a motor unit neurone.
  • Adjacent clean muscle skin cells are bound alongside one another at dense body, transmitting the contractile makes from cell to cell throughout the muscle.
  • Connective endomysium seprates cell

Function Differences

  • Smooth muscles cells are an involuntary action and can work slower so they do not have muscle tiredness.
  • Skeletal Muscle consists of both Fast & Slow Twitch muscle materials, that allow for a faster reaction where needed, and the contrary holds true for the Poor as well
  • Smooth muscle lines your arteries and airways and serves to written agreement or relax to help control blood pressure.
  • Skeletal muscles function almost continuously to maintain your pose making one small modification after another to keep the body upright
  • They are also within the iris of the attention to control the size of the pupil in response to light. Through the radial and circular muscle.
  • Skeletal muscle is also very important to holding your bones in the right position and helps prevent your joints from dislocating. Some skeletal muscles in that person are directly mounted on your skin
  • They set the GI tract to go "food"through the intestines. That is done by peristalsis.
  • Skeletal muscle generates high temperature as a by-product of muscle activity. This warmth is essential for maintaining your normal body temperature.
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