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Approach To Estimate The Salivary Circulation Using Chromatography


Aim: To build up a straightforward and safe solution to calculate the salivary move using chromatography paper.

Materials and Method: 30 volunteers participated in the study. The salivary move rate was predicted using chromatography filtration paper and compared with the salivary flow rate each and every minute. The assay contains 3 spots made up of starch and potassium iodide per spot on filter paper. Predicated on the color reaction on the chromatography filtration newspaper the salivary flow rate was approximated.

Results: Pearson's correlation test demonstrated highly significant negative relationship when the salivary stream rate and the number of coloured dots were compared.

Conclusion: This method can be utilized as a chairside diagnostic assist in estimation of salivary flow

Key words: chromatography filtration system paper, salivary move rate, diagnostic aid

A Novel Approach To Estimate The Salivary Circulation Using Chromatography Paper


Quantitative and/or qualitative modifications in salivary secretion can lead to localadverse results like caries, dental mucositis, candidiasis, oral infections, gnawing disorders, halitosis and useful undesireable effects like dysphagia, hypersalivation(sialorrhea) and hyposalivation(xerostomia). 1

Whole saliva (blended saliva) is a mixture of oral fluids that includes secretions from both the major and small salivary glands, in addition to many constituents of non-salivary origins, such as gingival crevicular liquid (GCF), expectoratedbronchial and nasal secretions, serum and bloodstream derivatives from dental wounds, bacteria and bacterial products, infections and fungi, desquamated epithelial cells, other cellular components, and food rubble. 2

Stimulated saliva is produced due to some mechanical, gustatory, olfactory, or pharmacological stimulus, adding to around 80% to 90% of daily salivary development.

In adults the full total stimulated salivary circulation varies from 1 to 3 ml/min3whereas the unstimulated salivary circulation at rest runs from 0. 25 to 0. 35 ml/min. 4

Unstimulated salivary move rate is most damaged by the degree of hydration, olfactory arousal, exposure to light, body setting, and seasonal and diurnal factors.

There are various options for the estimation of salivary circulation including way of measuring of glandular salivary stream, resting salivary circulation and stimulated salivary move.

The best two ways to acquire whole saliva are the draining method, in which saliva is permitted to drip off the low lip, and the spitting method, in which the subject matter expectorates saliva into a test pipe. 2

This method is frustrating and needs special equipment like collection tubes or amount meter.

AIM:To evaluatea simple and safe way for the estimation of salivary stream using chromatography paper


The study contains 30 volunteers comprising of 21 females and 9 men, aged 20-20yrs. The things were asked to remain in an upright position with the mind bent in a downward position and were asked to gather the saliva in their mouth for 1minute and asked to spit in to the collecting test pipes at the end of 1 1 minute to determine the salivary flow rate/min.


The filter newspaper for the estimation of salivary move was prepared corresponding to a way distributed by Takashi et al using soluble starch, potassium iodideand chromatography newspaper (70 mm 21 mm).

4l of the diagnosis reagent (1% starch solution and 0. 3 mol/L potassium iodide solution mixed at a proportion of 3:1) was positioned at 3 places on the chromatography newspaper using a micropipette.

The filter paper was left overnight in a very good dark room and then stored in a light-resistant container until use.

The soluble starch and potassium iodide were dissolved in 0. 1 mol/L Tris-HCl buffer the modified pH of 7. 3. 5


The coloring reagent was well prepared from a remedy of 31% hydrogen peroxide, ethanol and distilled drinking water at a ratio of 1 1:7:1.


Subjects were asked to open up their mouths and then to lift up the tongue.

The suggestion of the paper was put on the guts of the floor of the oral cavity in the sublingual region.

After 1 minute, the filtration paper was applied for and then your coloring reagent (around 2 L) was added dropwise to the areas in the filtration paper.

Via the iodine-starch reaction, colorless spots immediately switched blue.

The quantity of blue places, including partly shaded locations (50%), was grossly counted as an integer.

Based upon this we examined the partnership between the quantity of colored places and salivary flow rates after insertion of filtration system paper for 1 minute in the sublingual regions of healthy subjects. 5


Common methods for collecting entire saliva include draining, spitting, suction and swab (absorbent) method. In draining method saliva is allowed to drip off the lower lip into a preweighted pot or graduated test tube. In spitting method of collection, the saliva is permitted to collect in the ground of the mouth area and subject matter are asked to spit in the collecting pipes. Whereasin suction method saliva is consistently aspirated from the ground of the oral cavity into graduated test tubes. In absorbent method pre-weighed cotton rolls, swabs, or gauze are put into the beginning of the ductal orifices of the salivary glands and reweighed following the collection is completed. The suction and swab method triggers some extent of arousal and variability and therefore are not recommended for unstimulated salivary collection whereas swab method is said to be least reliable among all these methods. 6 the otherdisadvantages of these methods are that they are time consuming and need special equipment like collection pipes or size meter.

Takashi et al suggested the present method today's assay for salivary stream examination. The assay system consists of 3 locations 1mm apart filled with starch and potassium iodide on the filtration newspaper. Potassium iodide in the places easily gets displaced with the move of saliva, as opposed to this starch does not get displaced and gets maintained in the initial place. Thus, the colorless spots on the paper not infiltrated with saliva immediately switched blue with the help of the coloring reagent that included hydrogen peroxide, whereas the places on the newspaper infiltrated with saliva will not show the colour. Colored spot is based on the result of saliva between potassium iodide and starch in the chromatography paper and the colour result of iodine-starch to hydrogen peroxide. 5

Unstimulated saliva reflects the basal salivary move rate while stimulated salivarepresents the fuctional reserve of the salivary glands. Therefore the research of unstimulated saliva pays to for the study of salivary gland position. 1The presentmethod can be of assist in case of elderly patients suffering from xerostomia, in screening lab tests, post radiation therapy for tumors in elderly patients where salivary movement way of measuring can play a chair area diagnostic test.

In addition, the method can easily be tweaked to versions in cutoff prices and exactness by changing the amount of places and distance between your locations on the filtration paper

With the present method excellent results were obtained between your salivary stream rate and the colored spots with an r value of -1 and p value of 0. 0 in healthy individuals. Thus the routine use of this method as a couch side analysis assay for salivary stream would be of benefit to all training dentists and patients similarly.

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